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KMID : 0861020110260020045
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Korea Journal of Herbology
2011 Volume.26 No. 2 p.45 ~ p.49
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The Effects of Nelumbo nucifera on Utraviolet-B Irradiated human Keratinocytes
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Chang Mun-Seog
Ko Eun-Bit
Lee Ho-Jin
Kim Ju-Sung
Kim Jin-Soo
Jee Sung-Won
Kim Hyu-Young
Yeom Myeong-Hun
Kim Duck-Hee
Kim Han-Gon
Park Seong-Kyu
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Abstract
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Objectives: The purpose of this study was to investigate the anti-aging effects on cultured human keratinocytes with Nelumbo nucifera extracts.
Methods:Each parts of leaves, flowers and stamen were extracted with water or 70% ethanol. These extracts were tested for cell viability on HaCaT cells (human keratinocyte line) by MTT assay. We investigated the effects of Ultraviolet-B (UVB) irradiation on cytotoxicity and lipid peroxidation in cultured skin keratinocytes.
Results: The ethanol extract of Nelumbo nucifera flowers showed maximun cell viability as 111.39% in 30 ug/ml concentration. The water extracts of stamen, flowers, leaves showed cell viability as 107.12, 101.65, 101.46%, respectively. HaCaT keratinocytes were survived 63.06% at 20mJ/cm² UVB irradiation. The cell membrane lipid peroxidation was measured by accumulation malondialdehyde (MDA). The levels of MDA were decreased by the ethanol extract of Nelumbo nucifera flowers and the water extracts of stamen.
Conclusions :These finding suggest that the ethanol extract of Nelumbo nucifera flowers prevent anti-aging effects on cultured human keratinocytes during UVB irradiation.
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KEYWORD
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Ultraviolet-B (UVB), HaCaT keratinocyte, Nelumbo nucifera
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